Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Pharmacological evaluation of tacrolimus (FK-506) on ischemia reperfusion induced vasculatic neuropathic pain in rats. | Muthuraman and Sood Journal of Brachial Plexus and Peripheral Nerve Injury 2010 5 13 http content 5 1 13 JOURNAL OF BRACHIAL PLEXUS AND PERIPHERAL NERVE INJURY RESEARCH ARTICLE Open Access Phaermacological evaluation of tacrolimus FK-506 on ischemia reperfusion induced vasculatic neuropathic pain in rats Arunachalam Muthuraman and Shailja Sood Abstract Background Ischemia reperfusion I R is common in various pathological conditions like diabetic complication rheumatic arthritis necrotizing vascular occlusive disease and trauma. Methods We have evaluated the effect of tacrolimus 1 2 and 3 mg kg . for 10 consecutive days on femoral arterial ischemic reperfusion I R induced neuropathic pain in rats. Behavioral parameters . hot plate radiant heat acetone drop tail heat hyperalgesia tail flick and tail cold allodynia tests were assessed at different time intervals . 0 1 4 7 10 13 and 16th day and biochemical analysis in serum and tissue samples were also performed along with histopathological studies. Results Behavioral pain assessment revealed increase in the paw and tail withdrawal threshold in tacrolimus treated groups against hyperalgesic and allodynic stimuli as compared to the sham control group. We observed a decrease in the serum nitrate and thiobarbituric acid reactive substance TBARS levels along with reduction in tissue myeloperoxidase MPO and total calcium levels whereas rise in tissue reduced glutathione levels in tacrolimus treated groups. However significant results were obtained in medium and high dose treated group as compared to sham control group. Histopathological study had revealed the increase in the neuronal edema and axonal degeneration in the I R group whereas tacrolimus ameliorate these effects. Conclusion Our results indicate the anti-oxidative anti-inflammatory and calcium modulatory actions of tacrolimus. Therefore it can be used as a therapeutic agent for the treatment of vascular inflammatory related neuropathic .