Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells | Wang et al. Journal of Experimental Clinical Cancer Research 2010 29 159 http content 29 1 159 RESEARCH O1 It I Journal of Experimental Clinical Cancer Research Open Access Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells 1 1 1 1 3 1 Qiong Wang Xue-lian Zheng Lan Yang Fang Shi Lin-bo Gao Ying-jia Zhong Hong Sun Fan He Yong Lin1 Xia Wang1 Abstract Background Saikosaponin-a and -d two naturally occurring compounds derived from Bupleurum radix have been shown to exert anti-cancer activity in several cancer cell lines. However the effect of combination of saikosaponins with chemotherapeutic drugs has never been addressed. Thus we investigated whether these two saikosaponins have chemosensitization effect on cisplatin-induced cancer cell cytotoxicity. Methods Two cervical cancer cell lines HeLa and Siha an ovarian cancer cell line SKOV3 and a non-small cell lung cancer cell line A549 were treated with saikosaponins or cisplatin individually or in combination. Cell death was quantitatively detected by the release of lactate dehydrogenase LDH using a cytotoxicity detection kit. Cellular ROS was analyzed by flow cytometry. Apoptosis was evaluated by AO EB staining flow cytometry after Anexin V and PI staining and Western blot for caspase activation. ROS scavengers and caspase inhibitor were used to determine the roles of ROS and apoptosis in the effects of saikosaponins on cisplatin-induced cell death. Results Both saikosaponin-a and -d sensitized cancer cells to cisplatin-induced cell death in a dose-dependent manner which was accompanied with induction of reactive oxygen species ROS accumulation. The dead cells showed typical apoptotic morphologies. Both early apoptotic and late apoptotic cells detected by flow cytometry were increased in saikosaponins and cisplatin cotreated cells accompanied by activation of the caspase pathway. The pan-caspase .
