Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The HPB-AML-I cell line possesses the properties of mesenchymal stem cells | Journal of Experimental Clinical Cancer Research Ardianto et al. Journal of Experimental Clinical Cancer Research 2010 29 163 http content 29 1 163 RESEARCH Open Access The HPB-AML-I cell line possesses the properties of mesenchymal stem cells 2 r- 2 2 2 2 Bambang Ardianto 1 Takeshi Sugimoto Seiji Kawano Shimpei Kasagi Siti NA Jauharoh Chiyo Kurimoto Eiji Tatsumi3 Keiko Morikawa3 Shunichi Kumagai2 Yoshitake Hayashi1 Abstract Background In spite of its establishment from the peripheral blood of a case with acute myeloid leukemia AML -M1 HPB-AML-I shows plastic adherence with spindle-like morphology. In addition lipid droplets can be induced in HPB-AML-I cells by methylisobutylxanthine hydrocortisone and indomethacin. These findings suggest that HPB-AML-I is similar to mesenchymal stem cells MSCs or mesenchymal stromal cells rather than to hematopoietic cells. Methods To examine this possibility we characterized HPB-AML-I by performing cytochemical cytogenetic and phenotypic analyses induction of differentiation toward mesenchymal lineage cells and mixed lymphocyte culture analysis. Results HPB-AML-I proved to be negative for myeloperoxidase while surface antigen analysis disclosed that it was positive for MSC-related antigens such as CD29 CD44 CD55 CD59 and CD73 but not for CD14 CD19 CD34 CD45 CD90 CD105 CD117 and HLA-DR. Karyotypic analysis showed the presence of complicated abnormalities but no reciprocal translocations typically detected in AML cases. Following the induction of differentiation toward adipocytes chondrocytes and osteocytes HPB-AML-I cells showed in conjunction with extracellular matrix formation lipid accumulation proteoglycan synthesis and alkaline phosphatase expression. Mixed lymphocyte culture demonstrated that CD3 T-cell proliferation was suppressed in the presence of HPB-AML-I cells. Conclusions We conclude that HPB-AML-I cells appear to be unique neoplastic cells which may be derived from MSCs but are not hematopoietic .
