Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: MicroRNAs involved in neoplastic transformation of liver cancer stem cells | Journal of Experimental Clinical Cancer Research Li et al. Journal of Experimental Clinical Cancer Research 2010 29 169 http content 29 1 169 RESEARCH Open Access MicroRNAs involved in neoplastic transformation of liver cancer stem cells 11 2 11 11 1 1 Ren Li T Niansong Qian T Kaishan Tao T Nan You T Xinchuan Wang Kefeng Dou Abstract Background The existence of cancer stem cells in hepatocellular carcinoma HCC has been verified by characterizing side population SP cells based on efflux of Hoechst 33342 dye from stem cells. Recent advances in microRNA miRNA biology have revealed that miRNAs play an important role in embryonic development and tumorigenesis. However it is still unclear which miRNAs participate in the neoplastic transformation of liver cancer stem cells LCSCs during hepatocarcinogenesis. Methods To identify the unique set of miRNAs differentially regulated in LCSCs we applied SP sorting to primary cultures of F344 rat HCC cancer cells treated with diethylnitrosamine DEN and normal syngenic fetal liver cells and the stem-like characteristics of SP cells were verified through detecting expression of AFP and CK-7. Global miRNA expression profiles of two groups of SP cells were screened through microarray platform. Results A total of 68 miRNAs including miR-10b miR-21 miR-470 miR-34c-3p and let-7i were identified as overexpressed in SP of HCC cells compared to fetal liver cells. Ten miRNAs were underexpressed including miR-200a and miR-148b . These miRNAs were validated using stem-loop real-time reverse transcriptase polymerase chain reaction RT-PCR . Conclusions Our results suggest that LCSCs may have a distinct miRNA expression fingerprint during hepatocarcinogenesis. Dissecting these relationships will provide a new understanding of the function of miRNA in the process of neoplastic transformation of LCSCs. Background Cancer stem cells CSCs have been identified in hematopoietic malignancies and in solid tumors including .
