Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Microarray-based analysis of microRNA expression in breast cancer stem cells | Journal of Experimental Clinical Cancer Research Sun et al. Journal of Experimental Clinical Cancer Research 2010 29 174 http content 29 1 174 RESEARCH Open Access Microarray-based analysis of microRNA expression in breast cancer stem cells 1 2 11 1 1 11 Jian-guo Sun Rong-xia Liao Jun Qiu Jun-yu Jin Xin-xin Wang Yu-zhong Duan Fang-lin Chen Ping Hao Qi-chao Xie1 Zhi-xin Wang1 De-zhi Li1 Zheng-tang Chen1 Shao-xiang Zhang3 Abstract Background This study aimed to determine the miRNA profile in breast cancer stem cells BCSCs and to explore the functions of characteristic BCSC miRNAs. Methods We isolated ESA CD44 CD24- low BCSCs from MCF-7 cells using fluorescence-activated cell sorting FACS . A human breast cancer xenograft assay was performed to validate the stem cell properties of the isolated cells and microarray analysis was performed to screen for BCSC-related miRNAs. These BCSC-related miRNAs were selected for bioinformatic analysis and target prediction using online software programs. Results The ESA CD44 CD24- low cells had up to 100- to 1000-fold greater tumor-initiating capability than the MCF-7 cells. Tumors initiated from the ESA CD44 CD24- low cells were included of luminal epithelial and myoepithelial cells indicating stem cell properties. We also obtained miRNA profiles of ESA CD44 CD24- low BCSCs. Most of the possible targets of potential tumorigenesis-related miRNAs were oncogenes anti-oncogenes or regulatory genes. Conclusions We identified a subset of miRNAs that were differentially expressed in BCSCs providing a starting point to explore the functions of these miRNAs. Evaluating characteristic BCSC miRNAs represents a new method for studying breast cancer-initiating cells and developing therapeutic strategies aimed at eradicating the tumorigenic subpopulation of cells in breast cancer. Background Breast cancer is one of the most common cancers in women and poses a threat to women s health. Al-Hajj s research in 2003 has shown that .
