Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: RNAi-mediated knockdown of cyclooxygenase2 inhibits the growth, invasion and migration of SaOS2 human osteosarcoma cells: a case control study | Zhao et al. Journal of Experimental Clinical Cancer Research 2011 30 26 http content 30 1 26 Journal of Experimental Clinical Cancer Research RESEARCH Open Access RNAi-mediated knockdown of cyclooxygenase2 inhibits the growth invasion and migration of SaOS2 human osteosarcoma cells a case control study 1 2 1 1 1 f 71 2 1 Qinghua Zhao Chuan Wang Jiaxue Zhu Lei Wang Shuanghai Dong Guoqiao Zhang Jiwei Tian Abstract Background Cyclooxygenase2 COX-2 one isoform of cyclooxygenase proinflammatory enzymes is responsible for tumor development invasion and metastasis. Due to its role and frequent overexpression in a variety of human malignancies including osteosarcoma COX-2 has received considerable attention. However the function of COX-2 in the pathogenesis of cancer is not well understood. We examined the role of COX-2 in osteosarcoma. Methods We employed lentivirus mediated-RNA interference technology to knockdown endogenous gene COX-2 expression in human osteosarcoma cells SaOS2 and analyzed the phenotypical changes. The effect of COX-2 treatment on the proliferation cell cycle invasion and migration of the SaOS2 cells were assessed using the MTT flow cytometry invasion and migration assays respectively. COX-2 vascular endothelial growth factor VEGF epidermal growth factor EGF basic fibroblast growth factor bFGF mRNA and protein expression were detected by RT-PCR and western blotting. Results Our results indicate that a decrease of COX-2 expression in human osteosarcoma cells significantly inhibited the growth decreased the invasion and migration ability of SaOS2 cells. In addition it also reduced VEGF EGF and bFGF mRNA and protein expression. Conclusions The COX-2 signaling pathway may provide a novel therapeutic target for the treatment of human osteosarcoma. Background Osteosarcoma is the most common primary malignant tumor arising in bone predominantly affecting children and adolescents 1 . It is also one of the most heterogeneous of human tumors 2 . .
