Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Antisense oligodeoxynucleotides targeting ATM strengthen apoptosis of laryngeal squamous cell carcinoma grown in nude mice | Feng et al. Journal of Experimental Clinical Cancer Research 2011 30 43 http content 30 1 43 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Antisense oligodeoxynucleotides targeting ATM strengthen apoptosis of laryngeal squamous cell carcinoma grown in nude mice Jun Feng1 4t Jian Zou1t Li Li2 Yongsheng Zhao3 and Shixi Liu1 Abstract Background To conserve laryngeal function and elevate living quality of laryngeal squamous cell carcinoma LSCC patients we designed antisense oligodeoxynucleotides AS-ODNs to reduce expression of ATM and to enhance the apoptosis of hep-2 Human epidermoid laryngeal carcinoma cells to radiation in vitro and in vivo. Methods The expression of ATM mRNA and protein in hep-2 cells were examined by real-time quantitative PCR and western blotting respectively. Clonogenic survival assay was carried out to detect the survival ability of hep-2 cells after irradiation and analyzed the cell apoptosis by flow cytometry. The volume of solid tumors was measured while TUNEL assay and western blotting used to analyze cell apoptosis and protein expression after irradiation. Results The relative ATM mRNA and protein expression in hep-2 cells treated with ATM AS-ODNs were decreased to and of that in untreated cells respectively P . After irradiation the survival fraction SF of cells treated with ATM AS-ODNs was lower than that of other groups at the same dose of radiation P . The inhibition rate in hep-2 cells solid tumor exposed to X-ray alone was while it was in the group which irradiated in combination with the treatment of ATM AS-ODNs P . The apoptotic index for the group irradiated in combination with ATM AS-ODNs injection was which was significantly higher than that of others P . Conclusion AS-ODNs of ATM reduce ATM expression and enhance hep-2 cells apoptosis to radiation in vitro and in vivo. Keywords ATM Antisense oligodeoxynucleotides .
