Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Prognostic significance of STAT3 and phosphorylated STAT3 in human soft tissue tumors - a clinicopathological analysis | David et al. Journal of Experimental Clinical Cancer Research 2011 30 56 http content 30 1 56 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Prognostic significance of STAT3 and phosphorylated STAT3 in human soft tissue tumors - a clinicopathological analysis Diana David1 Lakshmy M Rajappan2 Krishna Balachandran3 Jissa V Thulaseedharan1 Asha S Nair1 and Radhakrishna M Pillai1 Abstract Background Signal transducer and activator of transcription 3 STAT3 is a key signaling molecule and a central cytoplasmic transcription factor implicated in the regulation of growth. Its aberrant activation has been demonstrated to correlate with many types of human malignancy. However whether constitutive STAT3 signaling plays a key role in the survival and growth of soft-tissue tumors is still unclear and hence needs to be elucidated further. In our study we examined the expression levels of STAT3 and pSTAT3 in different grades of soft tissue tumors and correlated with its clinicopathological characteristics. Methods Expression levels of STAT3 and pSTAT3 in soft tissue tumors were studied using Immunohistochemistry Western blotting and Reverse transcriptase- PCR and correlated with its clinicopathological characteristics using Chi squared or Fisher s exact test and by logistic regression analysis. Statistical analysis was done using Intercooled Stata software Intercooled Stata version . Results Of the 82 soft tissue tumor samples fifty four showed immunoreactivity for STAT3 and twenty eight for pSTAT3. Expression of STAT3 and pSTAT3 was significantly associated with tumor grade P P tumor location P P plane of tumor P P and tumor necrosis P P . Western blotting and RT-PCR analysis showed increased expression of STAT3 and p-STAT3 as grade of malignancy increased. Conclusion These findings suggest that constitutive activation of STAT3 is an important factor related to carcinogenesis of .
