Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Human serum-derived hydroxy long-chain fatty acids exhibit anti-inflammatory and anti-proliferative activity | Ritchie et al. Journal of Experimental Clinical Cancer Research 2011 30 59 http content 30 1 59 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Human serum-derived hydroxy long-chain fatty acids exhibit anti-inflammatory and anti-proliferative activity 1 1 11 Shawn A Ritchie Dushmanthi Jayasinghe Gerald F Davies Pearson Ahiahonu Hong Ma and Dayan B Goodenowe1 Abstract Background Circulating levels of novel long-chain hydroxy fatty acids called GTAs were recently discovered in the serum of healthy subjects which were shown to be reduced in subjects with colorectal cancer CRC independent of tumor burden or disease stage. The levels of GTAs were subsequently observed to exhibit an inverse association with age in the general population. The current work investigates the biological activity of these fatty acids by evaluating the effects of enriched human serum extracts on cell growth and inflammation. Methods GTAs were extracted from commercially available bulk human serum and then chromatographically separated into enriched GTA-positive and depleted GTA-negative fractions. SW620 MCF7 and LPS stimulated cells were treated with various concentrations of the GTA-positive and GTA-negative extracts and the effects on cell growth and inflammation determined. Results Enriched fractions resulted in poly-ADP ribose polymerase PARP cleavage suppression of NFkB induction of IftBa and reduction in NOS2 mRNA transcript levels. In mouse macrophage cells incubation with enriched fractions prior to treatment with LPS blocked the induction of several pro-inflammatory markers including nitric oxide TNFa IL-1P NOS2 and COX2. Conclusions Our results show that human serum extracts enriched with endogenous long-chain hydroxy fatty acids possess anti-inflammatory and anti-proliferative activity. These findings support a hypothesis that the reduction of these metabolites with age may result in a compromised ability to defend against .
