Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Small interference RNA targeting tissue factor inhibits human lung adenocarcinoma growth in vitro and in vivo | Xu et al. Journal of Experimental Clinical Cancer Research 2011 30 63 http content 30 1 63 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Small interference RNA targeting tissue factor inhibits human lung adenocarcinoma growth in vitro and in vivo 1 z- - -2 1 11 1 z- - z 1 1 Chengcheng Xu Qi Gui Wenshu Chen Leiming Wu Wei Sun Ni Zhang Qinzi Xu Jianing Wang and Xiangning Fu1 Abstract Background The human coagulation trigger tissue factor TF is overexpressed in several types of cancer and involved in tumor growth vascularization and metastasis. To explore the role of TF in biological processes of lung adenocarcinoma we used RNA interference RNAi technology to silence TF in a lung adenocarcinoma cell line A549 with high-level expression of TF and evaluate its antitumor effects in vitro and in vivo. Methods The specific small interfering RNA siRNA designed for targeting human TF was transfected into A549 cells. The expression of TF was detected by reverse transcription-PCR and Western blot. Cell proliferation was measured by MTT and clonogenic assays. Cell apoptosis was assessed by flow cytometry. The metastatic potential of A549 cells was determined by wound healing the mobility and Matrigel invasion assays. Expressions of PI3K Akt Erk1 2 VEGF and MMP-2 -9 in transfected cells were detected by Western blot. In vivo the effect of TF-siRNA on the growth of A549 lung adenocarcinoma xenografts in nude mice was investigated. Results TF -siRNA significantly reduced the expression of TF in the mRNA and protein levels. The down-regulation of TF in A549 cells resulted in the suppression of cell proliferation invasion and metastasis and induced cell apoptosis in dose-dependent manner. Erk MAPK PI3K Akt pathways as well as VEGF and MMP-2 -9 expressions were inhibited in TF-siRNA transfected cells. Moreover intratumoral injection of siRNA targeting TF suppressed the tumor growth of A549 cells in vivo model of lung adenocarcinoma. Conclusions .
