Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Salivary a-amylase exhibits antiproliferative effects in primary cell cultures of rat mammary epithelial cells and human breast cancer cells | Fedrowitz et al. Journal of Experimental Clinical Cancer Research 2011 30 102 http content 30 1 102 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Salivary a-amylase exhibits antiproliferative effects in primary cell cultures of rat mammary epithelial cells and human breast cancer cells Maren Fedrowitz1 Ralf Hass2 Catharina Bertram2 and Wolfgang Loscher1 Abstract Background Breast cancer is one of the most diagnosed cancers in females frequently with fatal outcome so that new strategies for modulating cell proliferation in the mammary tissue are urgently needed. There is some as yet inconclusive evidence that a-amylase may constitute a novel candidate for affecting cellular growth. Methods The present investigation aimed to examine if salivary a-amylase an enzyme well known for the metabolism of starch and recently introduced as a stress marker is able to exert antiproliferative effects on the growth of mammary gland epithelial cells. For this purpose primary epithelial cultures of breast tissue from two different inbred rat strains Fischer 344 F344 and Lewis as well as breast tumor cells of human origin were used. Treatment with human salivary a-amylase was performed once daily for 2 days followed by cell counting trypan blue assay to determine alterations in cell numbers. Cell senescence after a-amylase treatment was assessed by p-galactosidase assay. Endogenous a-amylase was detected in cells from F344 and Lewis by immunofluorescence. Results Salivary a-amylase treatment in vitro significantly decreased the proliferation of primary cells from F344 and Lewis rats in a concentration-dependent manner. Noticeably the sensitivity towards a-amylase was significantly higher in Lewis cells with stronger impact on cell growth after 5 and 50 U ml compared to F344 cells. An antiproliferative effect of a-amylase was also determined in mammary tumor cells of human origin but this effect varied depending on the donor age and type of the .
