Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Compound Kushen Injection suppresses human breast cancer stem-like cells by down-regulating the canonical Wnt/b-catenin pathway | Xu et al. Journal of Experimental Clinical Cancer Research 2011 30 103 http content 30 1 103 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Compound Kushen Injection suppresses human breast cancer stem-like cells by down-regulating the canonical Wnt p-catenin pathway 1 1 2 1 1 Z-S 1 -1 Weiru Xu Hongsheng Lin Ying Zhang Xinyi Chen Baojin Hua Wei Hou Xin Qi Yingxia Pei Xiaoyun Zhu3 Zhizheng Zhao1 and Liangliang Yang1 Abstract Background Cancer stem cells CSCs play an important role in cancer initiation relapse and metastasis. To date no specific medicine has been found to target CSCs as they are resistant to most conventional therapies and proliferate indefinitely. Compound Kushen Injection CKI has been widely used for cancer patients with remarkable therapeutic effects in Chinese clinical settings for many years. This study focused on whether CKI could inhibit MCF-7 SP cells in vitro and in vivo. Methods The analysis of CKI on SP population and the main genes of Wnt signaling pathway were studied first. Then we studied the tumorigenicity of SP cells and the effects of CKI on SP cells in vivo. The mice inoculated with 10 000 SP cells were randomly divided into three groups 6 in each group and treated with CKI cisplatin and saline as a control respectively for 7 weeks. The tumor formation rates of each group were compared. The main genes and proteins of the Wnt signaling pathway were analyzed by RT-PCR and western blot. Results CKI suppressed the size of SP population approximately 90 and down-regulated the main genes of Wnt signaling pathway. We also determined that MCF-7 SP cells were more tumorigenic than non-SP and unsorted cells. The Wnt signaling pathway was up-regulated in tumors derived from SP cells compared with that in tumors from non-SP cells. The tumor formation rate of the CKI Group was 33 2 6 P and that of Cisplatin Group was 50 3 6 P whereas that of the Control Group was 100 6 6 .The RT-PCR and western
