Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Ultrasound microbubble-mediated delivery of the siRNAs targeting MDR1 reduces drug resistance of yolk sac carcinoma L2 cells | He et al. Journal of Experimental Clinical Cancer Research 2011 30 104 http content 30 1 104 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Ultrasound microbubble-mediated delivery of the siRNAs targeting MDR1 reduces drug resistance of yolk sac carcinoma L2 cells 1 I_I Z 1 2 t VDi2 Vi I_I I I s 1 2 Ch z . r- i I II I 1 2 A Ỉ 1 2 I A Ỉ t z t 1 2 A A I z z i I I 1 2 I I 7 p. I I2 Yun He Yang Bi Yi Hua Dongyao Liu Sheng Wen Qiang Wang Mingyong LI Jing Zhu Tao Lin1 2 Dawei He1 2 Xuliang Li1 2 Zhigang Wang3 and Guanghui Wei1 2 Abstract Background MDR1 gene encoding P-glycoprotein is an ATP-dependent drug efflux transporter and related to drug resistance of yolk sac carcinoma. Ultrasound microbubble-mediated delivery has been used as a novel and effective gene delivery method. We hypothesize that small interfering RNA siRNA targeting MDR1 gene siMDR1 delivery with microbubble and ultrasound can down-regulate MDR1 expression and improve responsiveness to chemotherapeutic drugs for yolk sac carcinoma in vitro. Methods Retroviral knockdown vector pSEB-siMDR1s containing specific siRNA sites targeting rat MDR1 coding region were constructed and sequence verified. The resultant pSEB-siMDR1 plasmids DNA were encapsulated with lipid microbubble and the DNA release were triggered by ultrasound when added to culture cells. GFP positive cells were counted by flow cytometry to determine transfection efficiency. Quantitative real-time PCR and western blot were performed to determine the mRNA and protein expression of MDR1. P-glycoprotein function and drug sensitivity were analyzed by Daunorubicin accumulation and MTT assays. Results Transfection efficiency of pSEB-siMDR1 DNA was significantly increased by ultrasound microbubble-mediated delivery in rat yolk sac carcinoma L2 L2-RYC cells. Ultrasound microbubble-mediated siMDR1s delivery effectively inhibited MDR1 expression at both mRNA and protein levels and decreased P-glycoprotein function. .
