Dẫn truyền thần kinh cũng có thể điều chỉnh sự tích lũy ở gan của subpopulations một số tế bào lympho. Minagawa et al. [36] báo cáo rằng tiền xử lý với các thuốc đối kháng thụ thể adrenergic hầu như xóa bỏ sự tích tụ của các quần thể tế bào | CYTOKINES AND INFLAMMATORY RECRUITMENT IN NASH and certain types of liver lymphocytes including NKT cells express adrenergic receptors and respond to norepinephrine NE by producing various cytokines 32-35 . Neurotransmitters may also regulate the hepatic accumulation of certain lymphocyte subpopulations. Minagawa etal. 36 reported that pretreatment with adrenergic receptor antagonists virtually abolished the accumulation of NKT cell populations in the livers of mice that were subjected to partial hepatectomy. The latter finding intrigued us because ob ob mice are known to have both reduced NE levels and decreased hepatic NKT cells. Therefore we decided to evaluate the hypothesis that ob ob mice are sensitized to LPS hepatotoxicity because reduced NE inhibits the hepatic accumulation of NKT cells and results in Th-1 polarization of hepatic cytokine production in leptin-deficient mice. If NE proves to be a major proximal regulator of hepatic NKT cell populations then changes in NE activity may alter hepatic NKT cell numbers and influence hepatic cytokine production independently of leptin. This in turn suggests a mechanism for sensitization to LPS hepatotoxicity that may have general relevance to the pathogenesis of steatohepatitis. Norepinephrine increases hepatic NKT cells in leptin-deficient mice Because leptin deficiency induces multiple neuronal hormonal metabolic and immunological abnormalities including relative deficiency of NE it is difficult to predict which factors are predominately responsible for decreasing NKT cells in the livers of leptin-deficient mice. To assess the significance of NE deficiency to the hepatic depletion of NKT cells that occurs in leptin-deficient mice we implanted minipumps containing NE or saline vehicle subcutaneously into ob ob mice. Three weeks later hepatic mononuclear cells were isolated and fluorescent antibody cell sorting FACS analysis was performed to determine if NE altered hepatic mononuclear cell populations. NE .
