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báo cáo hóa học: " Differential aquaporin 4 expression during edema build-up and resolution phases of brain inflammation"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Differential aquaporin 4 expression during edema build-up and resolution phases of brain inflammation | Tourdias et al. Journal of Neuroinflammation 2011 8 143 http www.jneuroinflammation.eom content 8 1 143 JJOURNAL1 OF. NEUROINFLAMMATION RESEARCH Open Access Differential aquaporin 4 expression during edema build-up and resolution phases of brain inflammation 1.2 13 11 1 Thomas Tourdias Nobuyuki Mori Iulus Dragonu Nadège Cassagno Claudine Boiziau Justine Aussudre Bruno Brochet1 Chrit Moonen 3 Klaus G Petry13 and Vincent Dousset1 23 Abstract Background Vasogenic edema dynamically accumulates in many brain disorders associated with brain inflammation with the critical step of edema exacerbation feared in patient care. Water entrance through bloodbrain barrier BBB opening is thought to have a role in edema formation. Nevertheless the mechanisms of edema resolution remain poorly understood. Because the water channel aquaporin 4 AQP4 provides an important route for vasogenic edema resolution we studied the time course of AQP4 expression to better understand its potential effect in countering the exacerbation of vasogenic edema. Methods Focal inflammation was induced in the rat brain by a lysolecithin injection and was evaluated at 1 3 7 14 and 20 days using a combination of in vivo MRI with apparent diffusion coefficient ADC measurements used as a marker of water content and molecular and histological approaches for the quantification of AQP4 expression. Markers of active inflammation macrophages BBB permeability and interleukin-1b and markers of scarring gliosis were also quantified. Results This animal model of brain inflammation demonstrated two phases of edema development an initial edema build-up phase during active inflammation that peaked after 3 days ADC increase was followed by an edema resolution phase that lasted from 7 to 20 days post injection ADC decrease and was accompanied by glial scar formation. A moderate upregulation in AQP4 was observed during the build-up phase but a much stronger transcriptional and translational level of AQP4 expression was .