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Handbook of Microbiological Media, Fourth Edition part 6

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Handbook of Microbiological Media, Fourth Edition part 6 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Achromobacter Medium 45 PPLO broth without Crystal Violet.900.0mL Fresh yeast extract solution.100.0mL pH 7.8 0.2 at 25 C PPLO Broth without Crystal Violet Composition per 900.0mL Beef heart infusion from.225.0g Peptone.9.0g NaCl.4.5g Source PPLO broth without Crystal Violet is available as a premixed powder from BD Diagnostic Systems. Preparation of PPLO Broth without Crystal Violet Add components to distilled deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Fresh Yeast Extract Solution Composition per 100.0mL Baker s yeast live pressed starch-free.25.0g Preparation of Fresh Yeast Extract Solution Add the live Baker s yeast to 100.0mL of distilled deionized water. Mix thoroughly. Autoclave for 90 min at 15 psi pressure-121 C. Allow to stand. Remove supernatant solution. Adjust pH to 6.6-6.8. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into test tubes or flasks. Autoclave for 10 min at 15 psi pressure-121 C. Use For the cultivation and maintenance of Acholeplasma species. Acholeplasma Medium ATCC Medium 1215 Composition per 1020.0mL PPLO broth without Crystal Violet.700.0mL Fetal bovine serum heat inactivated.100.0mL Fresh yeast extract solution.100.0mL Tween -glucose-BSA solution.100.0mL Phenol Red 0.1 solution .20.0mL PPLO Broth without Crystal Violet Composition per 700.0mL Beef heart infusion from.175.0g Peptone. 7.0g NaCl.3.5g Source PPLO broth without Crystal Violet is available as a premixed powder from BD Diagnostic Systems. Preparation of PPLO Broth without Crystal Violet Add components to distilled deionized water and bring volume to 700.0mL. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Fresh Yeast Extract Solution Composition per 100.0mL Baker s yeast live pressed starch-free.25.0g Preparation of Fresh Yeast Extract Solution Add the .