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Handbook of Microbiological Media, Fourth Edition part 12

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Handbook of Microbiological Media, Fourth Edition part 12 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Anaerobic HiVeg Agar Base with Egg Yolk Emulsion 105 Preparation of Medium Add components except egg yolk emulsion to distilled deionized water and bring volume to 920.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add sterile egg yolk emulsion. Mix thoroughly. Pour into sterile Petri dishes. Use For the cultivation of Clostridium perfringens from foods. Anaerobic D-Gluconate Medium Composition per liter Agar.15.0g Pancreatic digest of casein.10.0g Yeast extract.5.0g D-Gluconate.4.0g MgSO4-7H2O.2.5g NH4 2SÛ4.1.4g L-Cysteine-HCl-H2O.1.0g CaCl22H2O.0.15g FeSO4-7H2O.0.02g Resazurin.1.0mg NaHCO3 solution.10.0mL pH 7.1 0.2 at 25 C NaHCO3 Solution Composition per 100.0mL NaHCO3.10.0g Preparation of NaHCO3 Solution Add NaHCO3 to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except NaHCO3 solution to distilled deionized water and bring volume to 990.0mL. Prepare anaerobically under 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add 10.0mL of the sterile NaHCO3 solution. Mix thoroughly. Adjust pH to 7.1. Use For the cultivation and maintenance of microorganisms that can utilize D-gluconate as a carbon source such as Bacteroides pectinophi-lus. Anaerobic Glucuronic Acid Medium Composition per liter Agar.15.0g Pancreatic digest of casein.10.0g Yeast extract.5.0g Glucuronic acid.4.0g MgSO4-7H2O.2.5g NH4 2SO4. 1.4g L-Cysteine-HCl-H2O.1.0g CaCl22H2O.0.15g FeSO4-7H2O.0.02g Resazurin . 1.0mg NaHCO3 solution.10.0mL pH 7.1 0.2 at 25 C NaHCO3 Solution Composition per 100.0mL NaHCO3.10.0g Preparation of NaHCO3 Solution Add NaHCO3 to distilled de-ionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except NaHCO3 solution to distilled deionized water and bring volume to 990.0mL. Pre pare anaerobically under 100 N2. Autoclave for 15 min at 15 psi