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Báo cáo y học: "Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài:Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration. | Available online http arthritis-research.eom content 11 3 R65 Research article Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells implications for the pathogenesis of intervertebral disc degeneration S Jane Millward-Sadler Patrick W Costello Anthony J Freemont and Judith A Hoyland Tissue Injury and Repair Group School of Clinical and Laboratory Sciences Faculty of Human and Medical Sciences University of Manchester Stopford Building Oxford Road Manchester M13 9PT UK Corresponding author Judith A Hoyland Judith.Hoyland@manchester.ac.uk Received 27 Nov 2008 Revisions requested 20 Jan 2009 Revisions received 8 Mar 2009 Accepted 12 May 2009 Published 12 May 2009 Arthritis Research Therapy 2009 11 R65 doi 10.1186 ar2693 This article is online at http arthritis-research.com content 11 3 R65 2009 Millward-Sadler et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction The aim of this study was to compare the effects of tumour necrosis factor-alpha TNF-a and interleukin-1-beta IL-1p on protease and catabolic cytokine and receptor gene expression in normal and degenerate human nucleus pulposus cells in alginate culture. Methods Cells isolated from normal and degenerate nucleus pulposus regions of human intervertebral discs were cultured in alginate pellets and stimulated by the addition of 10 ng mL TNF-a or IL-1 p for 48 hours prior to RNA extraction. Quantitative realtime polymerase chain reaction was used to assess the effect of TNF-a or IL-p stimulation on the expression of matrix metalloproteinase MMP -3 -9 and -13 TNF-a TNF receptor 1 TNF-R1 TNF receptor 2 TNF-R2 IL-1 a IL-1p IL-1 receptor 1 IL-1R1 and IL-1 receptor antagonist IL-1Ra . Results MMP-3 .