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Báo cáo y học: " Cyclophilin A interacts with diverse lentiviral capsids"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Cyclophilin A interacts with diverse lentiviral capsids. | Retrovirology BioMed Central Research Cyclophilin A interacts with diverse lentiviral capsids Tsai-Yu Lin1 and Michael Emerman 1 2 Open Access Address 1Pathobiology Graduate Program University of Washington Seattle WA 98195 USA and 2Division of Human Biology Fred Hutchinson Cancer Research Center Seattle WA 98109 USA Email Tsai-Yu Lin - linty@u.washington.edu Michael Emerman - memerman@fhcrc.org Corresponding author Published 12 October 2006 Received 27 July 2006 Accepted 12 October 2006 Retrovirology 2006 3 70 doi 10.1186 1742-4690-3-70 This article is available from http www.retrovirology.cOm content 3 1 70 2006 Lin and Emerman licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The capsid CA protein of HIV-1 binds with high affinity to the host protein cyclophilin A CypA . This binding positively affects some early stage of the viral life-cycle because prevention of binding either by drugs that occupy that active site of cyclophilin A by mutation in HIV-1 CA or RNAi that knocks down intracellular CypA level diminishes viral infectivity. The closely related lentivirus SIVcpz also binds CypA but it was thought that this interaction was limited to the HIV-1 SIVcpz lineage because other retroviruses failed to interact with CypA in a yeast two-hybrid assay. Results We find that diverse lentiviruses FIV and SIVagmTAN also bind to CypA. Mutagenesis of FIV CA showed that an amino acid that is in a homologous position to the proline at amino acid 90 of HIV-1 CA is essential for FIV interactions with CypA. Conclusion These results demonstrate that CypA binding to lentiviruses is more widespread than previously thought and .