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Báo cáo y học: " Overlapping enhancer/promoter and transcriptional termination signals in the lentiviral long terminal repeat"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài:" Overlapping enhancer/promoter and transcriptional termination signals in the lentiviral long terminal repeat. | Retrovirology BioMed Central Open Access Short report Overlapping enhancer promoter and transcriptional termination signals in the lentiviral long terminal repeat Qing Yang Aurore Lucas Sodany Son and Lung-Ji Chang Address Department of Molecular Genetics and Microbiology Powell Gene Therapy Center and McKnight Brain Institute University of Florida College of Medicine Gainesville Florida 32606 USA Email Qing Yang - qyang@ufl.edu Aurore Lucas - lucas.aurore@caramail.com Sodany Son - Sodany_Son@chiron.com Lung- Ji Chang - lchang@mgm.ufl.edu Corresponding author Published 22 January 2007 Received 2 October 2006 Accepted 22 January 2007 Retrovirology 2007 4 4 doi 10.1186 1742-4690-4-4 p This article is available from http www.retrovirology.com content 4 1 4 2007 Yang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.Org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Oncoretrovirus but not lentivirus displays a high transcriptional readthrough activity in the 3 long terminal repeat LTR Zaiss et al. J. Virol. 76 7209-7219 2002 . However the U3-deleted selfinactivating SIN lentiviral LTR also exhibits high transcriptional readthrough activity. Since the canonical core polyadenylation signal AAUAAA of the lentivirus is located in the R-U5 region the above finding suggests that additional RNA termination signals must be present in the U3 region. Insertion of alternative termination signals including panhuman T cell leukemia virus type I polyadenylation signal a 3 end small intron and a tertiary tRNA motif into the lentiviral SIN LTR did not restore the transcriptional termination function. Functional dissection of the U3 region revealed that 70-80 of the termination signals reside in the transcriptional control region within 124 nt overlapping NFkB Sp1 and TATA binding