Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity | Durán et al. Journal of Nanobiotechnology 2011 9 47 http content 9 1 47 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids efficiency and cytotoxicity 1 f 2Ỷ 3 1 2 María Carolina Durán Saskia Willenbrock Annette Barchanski Jessika-M V Muller Arianna Maiolini I s u . I c I I V4 c 4 I o vr I A ỉf 1 15 I z l I l z 2 If I z-i z- 1 I Il K II iri I s c P. S r2 Jan T Soller Stephan Barcikowski Ingo Nolte Karsten Feige and Hugo Murua Escobar Abstract Background Reproducibly high transfection rates with low methodology-induced cytotoxic side effects are essential to attain the required effect on targeted cells when exogenous DNA is transfected. Different approaches and modifications such as the use of nanoparticles NPs are being evaluated to increase transfection efficiencies. Several studies have focused on the attained transfection efficiency after NP-mediated approaches. However data comparing toxicity of these novel approaches with conventional methods is still rare. Transfection efficiency and methodology-induced cytotoxicity were analysed after transfection with different NP-mediated and conventional approaches. Two eukaryotic DNA-expression-plasmids were used to transfect the mammalian cell line MTH53A applying six different transfection protocols conventional transfection reagent FuGENE HD FHD FHD in combination with two different sizes of stabilizer-free laser-generated AuNPs PLAL-AuNPs_S1 _S2 FHD and commercially available AuNPs Plano-AuNP and two magnetic transfection protocols. 24 h post transfection efficiency of each protocol was analysed using fluorescence microscopy and GFP-based flow cytometry. Toxicity was assessed measuring cell proliferation and percentage of propidium iodide PI positive cells. Expression of the respective recombinant proteins was evaluated by immunofluorescence. Results The addition of AuNPs to the transfection protocols .
