Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Specific antibody response of mice after immunization with COS-7 cell derived avian influenza virus (H5N1) recombinant proteins. | Journal of Immune Based Therapies and Vaccines Original research BioMed Central Open Access Specific antibody response of mice after immunization with COS-7 cell derived avian influenza virus H5N1 recombinant proteins Navin Horthongkham1 Tananun Srihtrakul1 Niracha Athipanyasilp1 Sontana Siritantikorn1 Wannee Kantakamalakul1 Yong Poovorawan2 and Ruengpung Sutthent 1 Address Department of Microbiology Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand and 2Department of Pediatric Faculty of Medicine Chulalongkorn University Bangkok Thailand Email Navin Horthongkham - navmoo@ Tananun Srihtrakul - tanadew@ Niracha Athipanyasilp - niracha_19@ Sontana Siritantikorn - sissn@ Wannee Kantakamalakul - siwkk@ Yong Poovorawan - Ruengpung Sutthent - sirst@ Corresponding author Published 3 October 2007 Received 7 March 2007 Journal of Immune Based Therapies and Vaccines 2007 5 10 doi 1476-8518-5-10 Accepted 3 October 2007 This article is available from http content 5 1 10 2007 Horthongkham et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract To develop avian influenza H5N1 recombinant protein the hemagglutinin HA neuraminidase NA matrix M and non-structural NS1 of avian influenza H5N1 isolates from Thailand were engineered to be expressed in prokaryotic E. coli and mammalian cell COS-7 system. The plasmid pBAD-His and pSec-His were used as vectors for these inserted genes. Mice immunized with purified recombinant proteins at concentration 50-250 pg intramuscularly with Alum adjuvant at week 0 week 2 and week 3 showed a good immunogenicity measured by ELISA and neutralization assay. The HA and
