Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Transcriptional analysis of late ripening stages of grapevine berry | Guillaumie et al. BMC Plant Biology 2011 11 165 http 1471-2229 11 165 BMC Plant Biology RESEARCH ARTICLE Open Access Transcriptional analysis of late ripening stages of grapevine berry 12 s I- z- 12 3 Sabine Guillaumie Romain Fouquet Christian Kappel Céline Camps Nancy Terrier Driminim IO k l ìnr-rimhc4 17 1 0 Pi Pl inlo 5 ChridTinhcir Ptỉ Ìoc6 il 14 R iCC6 2nd forziQ Polrm 1 2 Dominique Moncomble Jake D Dunlevy Cinistopiiei Davies Paul K Boss and Seige Denot Abstract Background The composition of grapevine berry at harvest is a major determinant of wine quality. Optimal oenological maturity of berries is characterized by a high sugar acidity ratio high anthocyanin content in the skin and low astringency. However harvest time is still mostly determined empirically based on crude biochemical composition and berry tasting. In this context it is interesting to identify genes that are expressed repressed specifically at the late stages of ripening and which may be used as indicators of maturity. Results Whole bunches and berries sorted by density were collected in vineyard on Chardonnay white cultivar grapevines for two consecutive years at three stages of ripening 7-days before harvest TH-7 harvest TH and 10days after harvest TH 10 . Microvinification and sensory analysis indicate that the quality of the wines made from the whole bunches collected at TH-7 TH and TH 10 differed TH providing the highest quality wines. In parallel gene expression was studied with Qiagen Operon microarrays using two types of samples . whole bunches and berries sorted by density. Only 12 genes were consistently up- or down-regulated in whole bunches and density sorted berries for the two years studied in Chardonnay. 52 genes were differentially expressed between the TH-7 and TH samples. In order to determine whether these genes followed a similar pattern of expression during the late stages of berry ripening in a red cultivar nine genes were selected for .
