Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Japanese Encephalitis Virus wild strain infection suppresses dendritic cells maturation and function, and causes the expansion of regulatory T cells | Cao et al. Virology Journal 2011 8 39 http content 8 1 39 J VIROLOGY JOURNAL RESEARCH Open Access Japanese Encephalitis Virus wild strain infection suppresses dendritic cells maturation and function and causes the expansion of regulatory T cells 13 Shengbo Cao Yaoming Li Jing Ye Xiaohong Yang Long Chen Xueqin Liu Huanchun Chen Abstract Background Japanese encephalitis JE caused by Japanese encephalitis virus JEV accounts for acute illness and death. However few studies have been conducted to unveil the potential pathogenesis mechanism of JEV. Dendritic cells DCs are the most prominent antigen-presenting cells APCs which induce dual humoral and cellular responses. Thus the investigation of the interaction between JEV and DCs may be helpful for resolving the mechanism of viral escape from immune surveillance and JE pathogenesis. Results We examined the alterations of phenotype and function of DCs including bone marrow-derived DCs bmDCs in vitro and spleen-derived DCs spDCs in vivo due to JEV P3 wild strain infection. Our results showed that JEV P3 infected DCs in vitro and in vivo. The viral infection inhibited the expression of cell maturation surface markers CD40 CD80 and CD83 and MHCI and impaired the ability of P3-infected DCs for activating allogeneic naive T cells. In addition P3 infection suppressed the expression of interferon IFN -a and tumor necrosis factor TNF -a but enhanced the production of chemokine C-C motif ligand 2 CCL2 and interleukin IL -10 of DCs. The infected DCs expanded the population of CD4 Foxp3 regulatory T cell Treg . Conclusion JEV P3 infection of DCs impaired cell maturation and T cell activation modulated cytokine productions and expanded regulatory T cells suggesting a possible mechanism of JE development. Background JEV is a causative agent of JE which causes at least 50 000 clinical cases and about 10 000 deaths each year. It is a member of the mosquito-borne encephalitis complex of the .
