Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Recombinant luciferase-expressing human cytomegalovirus (CMV) for evaluation of CMV inhibitors | He et al. Virology Journal 2011 8 40 http content 8 1 40 J VIROLOGY JOURNAL RESEARCH Open Access Recombinant luciferase-expressing human cytomegalovirus CMV for evaluation of CMV inhibitors 1 2 2 2 3 4 1 Ran He Gordon Sandford Gary S Hayward William H Burns Gary H Posner Michael Forman Ravit Arav-Boger Abstract Recombinant Towne CMV expressing luciferase under the control of CMV-DNA polymerase POL or the late pp28 UL99 promoters were evaluated for potential application in high-throughput screening of anti-viral compounds. POL-and pp28-luciferase displayed maximal expression 48 and 72 hours post infection respectively. The pp28-luciferase virus achieved a wider dynamic range of luciferase expression 6-7 logs and was selected for testing of inhibition by five anti-viral compounds. Luciferase expression highly correlated with plaque reduction and real-time PCR. The pp28-luciferase reporter system is rapid reproducible and highly sensitive. It may be applied to screening of novel anti-CMV compounds. Background Infection with Cytomegalovirus CMV continues to be a major threat in organ transplant recipients and congenitally-infected children 1 2 . Although existing systemic therapies are effective in suppressing virus replication serious side effects and the emergence of resistant viral strains pose significant treatment dilemmas 3 . The need to identify and develop new anti-CMV compounds coincides with the advancement of rapid sensitive and high-throughput methods for screening of lead compounds. While the plaque reduction assay remains the gold-standard for screening of anti-viral compounds new techniques based on recombinant DNA technology and highly sensitive molecular assays have recently been suggested as alternatives 4-6 . Real-time PCR the standard of care in the management of CMV disease in high- risk patient populations may also provide a sensitive tool for drug screening 7-12 In earlier studies a series of chloramphenicol acetyl transferase .
