báo cáo khoa học: " A saturated SSR/DArT linkage map of Musa acuminata addressing genome rearrangements among bananas"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A saturated SSR/DArT linkage map of Musa acuminata addressing genome rearrangements among bananas | Hippolyte et al. BMC Plant Biology 2010 10 65 http 1471-2229 10 65 BMC Plant Biology RESEARCH ARTICLE _ Open Access A saturated SSR DArT linkage map of Musa acuminata addressing genome rearrangements among bananas Isabelle Hippolyte 1 Frederic Bakry1 Marc Seguin2 Laetitia Gardes2 3 Ronan Rivallan2 Ange-Marie Risterucci2 Christophe Jenny4 Xavier Perrier1 Frangoise Carreel5 Xavier Argout2 Pietro Piffanelli2 6 Imtiaz A Khan7 Robert NG Miller8 Georgios J Pappas9 Didier Mbéguié-A-Mbéguié10 Takashi Matsumoto11 Veronique De Bernardinis12 Eric Huttner13 Andrzej Kilian13 Franc-Christophe Baurens2 Angélique D Hont2 Frangois Cote14 Brigitte Courtois2 and Jean-Christophe Glaszmann2 Abstract Background The genus Musa is a large species complex which includes cultivars at diploid and triploid levels. These sterile and vegetatively propagated cultivars are based on the A genome from Musa acuminata exclusively for sweet bananas such as Cavendish or associated with the B genome Musabalbisiana in cooking bananas such as Plantain varieties. In M. acuminata cultivars structural heterozygosity is thought to be one of the main causes of sterility which is essential for obtaining seedless fruits but hampers breeding. Only partial genetic maps are presently available due to chromosomal rearrangements within the parents of the mapping populations. This causes large segregation distortions inducing pseudo-linkages and difficulties in ordering markers in the linkage groups. The present study aims at producing a saturated linkage map of M. acuminata taking into account hypotheses on the structural heterozygosity of the parents. Results An F1 progeny of 180 individuals was obtained from a cross between two genetically distant accessions of M. acuminata Borneo and Pisang Lilin P. Lilin . Based on the gametic recombination of each parent two parental maps composed of SSR and DArT markers were established. A significant proportion of the markers deviated p from the

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