Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Multiplex Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR) for diagnosis of natural infection with canine distemper virus | Chulakasian et al. Virology Journal 2010 7 122 http content 7 1 122 VIROLOGY JOURNAL RESEARCH Open Access Multiplex Amplification Refractory Mutation System Polymerase Chain Reaction ARMS-PCR for diagnosis of natural infection with canine distemper virus Songkhla Chulakasian 1 Min-Shiuh Lee2 Chi-Young Wang1 Shyan-Song Chiou3 Kuan-Hsun Lin1 Fong-Yuan Lin1 Tien-Huan Hsu1 Min-Liang Wong1 Tien-Jye Chang 1 and Wei-Li Hsu 3 Abstract Background Canine distemper virus CDV is present worldwide and produces a lethal systemic infection of wild and domestic Canidae. Pre-existing antibodies acquired from vaccination or previous CDV infection might interfere the interpretation of a serologic diagnosis method. In addition due to the high similarity of nucleic acid sequences between wild-type CDV and the new vaccine strain current PCR derived methods cannot be applied for the definite confirmation of CD infection. Hence it is worthy of developing a simple and rapid nucleotide-based assay for differentiation of wild-type CDV which is a cause of disease from attenuated CDVs after vaccination. High frequency variations have been found in the region spanning from the 3 -untranslated region UTR of the matrix M gene to the fusion F gene designated M-F UTR in a few CDV strains. To establish a differential diagnosis assay an amplification refractory mutation analysis was established based on the highly variable region on M-F UTR and F regions. Results Sequences of frequent polymorphisms were found scattered throughout the M-F UTR region the identity of nucleic acid between local strains and vaccine strains ranged from to . A track of AAA residue located 35 nucleotides downstream from F gene start codon highly conserved in three vaccine strains were replaced with TGC in the local strains that severed as target sequences for deign of discrimination primers. The method established in the present study successfully differentiated seven Taiwanese CDV field isolates
