Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo | Virology Journal BioMed Central Open Access Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo Jin-Long Yang1 2 An-Chun Cheng 2 3 Ming-Shu Wang2 3 Kang- Cheng Pan2 3 Min Li2 Yu-Fei Guo2 Chuan-Feng Li2 De-Kang Zhu2 3 and Xiao-Yue Chen2 3 Address 1Chongqing Academy of Animal Science Chongqing 402460 Chongqing China 2Avian Diseases Research Center College of Veterinary Medicine of Sichuan Agricultural University Yaan 625014 Sichuan China and 3Key Laboratory of Animal Diseases and Human Health of Sichuan Province Yaan 625014 Sichuan Province China Email Jin-Long Yang - yjlfirst@ An-Chun Cheng - chenganchun@ Ming-Shu Wang - mshwang@ Kang-Cheng Pan - pankangcheng71@ Min Li - loadstar@ Yu-Fei Guo - gyf02@ Chuan-Feng Li - lichuanfeng@ De-KangZhu-zdk24@ Xiao-Yue Chen-chenxiaoyue@ Corresponding author Published 15 September 2009 Received 7 July 2009 Accepted 15 September 2009 Virologyjournal 2009 6 142 doi 1743-422X-6-142 This article is available from http content 6 1 142 2009 Yang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Goose parvovirus GPV is a Dependovirus associated with latent infection and mortality in geese. Currently it severely affects geese production worldwide. The objective of this study was to develop a fluorescent quantitative real-time polymerase chain reaction PCR FQ-PCR assay for fast and accurate quantification of GPV DNA in infected goslings which can aid in the understanding of the regular distribution pattern and the nosogenesis of GPV in vivo. Results The detection limit of the assay was X 101
