Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso | Tiendrébéogo et al. Virology Journal 2010 7 48 http content 7 1 48 VIROLOGY JOURNAL SHORT REPORT Open Access Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso Fidèle Tiendrébéogo1 2 Pierre Lefeuvre2 Murielle Hoareau2 Julie Villemot2 Gnissa Konaté3 Alfred S Traoré1 Nicolas Barro1 Valentin S Traoré3 Bernard Reynaud2 Oumar Traoré3 Jean-Michel Lett2 Abstract Okra leaf curl disease OLCD is a major constraint on okra Abelmoschus esculentus production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso BF we show that the disease is associated with a monopartite begomovirus and satellite DNA complexes. Twenty-three complete genomic sequences of Cotton leaf curl Gezira virus CLCuGV isolates associated with OLCD sharing 95 to 99 nucleotide identity were cloned and sequenced. Six betasatellite and four alphasatellite DNA-1 molecules were also characterized. The six isolates of betasatellite associated with CLCuGV isolates correspond to Cotton leaf curl Gezira betasatellite CLCuGB 88 to 98 nucleotide identity . One isolate of alphasatellite is a variant of Cotton leaf curl Gezira alphasatellite CLCuGA 89 nucleotide identity whereas the three others isolates appear to correspond to a new species of alphasatellite CLCuGA most similar sequence present 52 to 60 nucleotide identity provisionally named Okra leaf curl Burkina Faso alphasatellite OLCBFA . Recombination analysis of the viruses demonstrated the interspecies recombinant origin of all CLCuGV isolates with parents being close to Hollyhock leaf crumple virus AY036009 and Tomato leaf curl Diana virus AM701765 . Combined with the presence of satellites DNA these results highlight the complexity of begomoviruses associated with OLCD. Findings Okra leaf curl disease OLCD is commonly observed among okra Abelmoschus esculentus crops in Burkina Faso BF .
