Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Development of real time PCR for detection and quantitation of Dengue Viruses | Virology Journal BioMed Central Open Access Methodology Development of real time PCR for detection and quantitation of Dengue Viruses KR Gurukumar D Priyadarshini JA Patil A Bhagat A Singh PS Shah and D Cecilia Address National Institute of Virology 20A Dr. Ambedkar Road Pune 411001 India Email KR Gurukumar - krgiyer@ D Priyadarshini - priya_darshini_d@ JA Patil - jayashriniv@ A Bhagat - ashabhagat4u@ A Singh - anand_singh50@ PS Shah - paresh17@ D Cecilia - cdayaraj@ Corresponding author Published 23 January 2009 Received 12 September 2008 Accepted 23 January 2009 Virology Journal 2009 6 10 doi l 743-422X-6-10 This article is available from http content 6 1 10 2009 Gurukumar et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Dengue virus DENV a mosquito borne flavivirus is an important pathogen causing more than 50 million infections every year around the world. Dengue diagnosis depends on serology which is not useful in the early phase of the disease and virus isolation which is laborious and time consuming. There is need for a rapid sensitive and high throughput method for detection of DENV in the early stages of the disease. Several real-time PCR assays have been described for dengue viruses but there is scope for improvement. The new generation TaqMan Minor Groove Binding MGB probe approach was used to develop an improved real time RT-PCR qRT-PCR for DENV in this study. Results The 3 UTR of thirteen Indian strains of DENV was sequenced and aligned with 41 representative sequences from GenBank. A region conserved in all four serotypes was used to target primers and probes for the qRT-PCR. A single MGB
