Avian influenza virus (H5N1); effects of physico-chemical factors on its survival | Virology Journal BioMed Central Research Avian influenza virus H5N1 effects of physico-chemical factors on its survival Muhammad Akbar Shahid1 Muhammad Abubakar 2 Sajid Hameed3 and Shamsul Hassan1 Open Access Address Poultry Research Institute Shamsabad Murree road Punjab Rawalpindi Pakistan 2National Veterinary Laboratory Park road Islamabad Pakistan and 3University College of Veterinary and Animal Sciences The Islamia University of Bahawalpur Bahawalpur Pakistan Email Muhammad Akbar Shahid - drakbarshahid@ Muhammad Abubakar - hayee41@ Sajid Hameed - sajidkumboh@ Shamsul Hassan - dprirwp@ Corresponding author Published 28 March 2009 Received 5 February 2009 Accepted 28 March 2009 Virology Journal 2009 6 38 doi l 743-422X-6-38 This article is available from http content 6 1 38 2009 Shahid et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Present study was performed to determine the effects of physical and chemical agents on infective potential of highly pathogenic avian influenza HPAI H5N1 local strain virus recently isolated in Pakistan during 2006 outbreak. H5N1 virus having titer 108-3 ELD50 ml was mixed with sterilized peptone water to get final dilution of 4HA units and then exposed to physical temperature pH and ultraviolet light and chemical formalin phenol crystals iodine crystals CID 20 virkon -S zeptin 10 KEPCIDE 300 KEPCIDE 400 lifebuoy surf excel and caustic soda agents. Harvested amnio-allantoic fluid AAF from embryonated chicken eggs inoculated with H5N1 treated virus ml egg was subjected to haemagglutination HA and haemagglutination inhibition HI tests. H5N1 virus lost infectivity after 30 min at 56 C after 1 day at 28 C but .
