Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A flax fibre proteome: identification of proteins enriched in bast fibres | BMC Plant Biology BioMed Central Research article Open Access A flax fibre proteome identification of proteins enriched in bast fibres Naomi SC Hotte and Michael K Deyholos Address Department of Biological Sciences Edmonton T6G 2E9 Canada Email Naomi SC Hotte - nhotte@ Michael K Deyholos - deyholos@ Corresponding author Published 30 April 2008 Received 7 November 2007 BMC Plant Biology 2008 8 52 doi 1471-2229-8-52 Accepted 30 April 2008 This article is available from http 1471-2229 8 52 2008 Hotte and Deyholos licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Bast fibres from the phloem tissues of flax are scientifically interesting and economically useful due in part to a dynamic system of secondary cell wall deposition. To better understand the molecular mechanisms underlying the process of cell wall development in flax we extracted proteins from individually dissected phloem fibres . individual cells at an early stage of secondary cell wall development and compared these extracts to protein extracts from surrounding non-fibre cells of the cortex using fluorescent DiGE labels and 2D-gel electrophoresis with identities assigned to some proteins by mass spectrometry. Results The abundance of many proteins in fibres was notably different from the surrounding non-fibre cells of the cortex with approximately 13 of the 1 850 detectable spots being significantly fold p enriched in fibres. Following mass spectrometry we assigned identity to 114 spots of which 51 were significantly enriched in fibres. We observed that a K channel subunit annexins porins secretory pathway components P-amylase P-galactosidase and pectin and galactan biosynthetic .
