Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Anti-angiogenic effect of high doses of ascorbic acid | Journal of Translational Medicine BioMed Central Open Access Anti-angiogenic effect of high doses of ascorbic acid Nina A Mikirova t1 Thomas E Ichimt2 and Neil H Riordant2 Address Bio-Communications Research Institute Wichita Kansas USA and 2Medistem Laboratories Inc Chandler Arizona USA Email Nina A Mikirova - nmikirova@ Thomas E Ichim - Neil H Riordan - riordan@ Corresponding author tEqual contributors Published 12 September 2008 Received 22 May 2008 . . rn J. 1 I mz 1 z-rn rn-rz z Accepted 12 September 2008 Journal of Translational Medicine 2008 6 50 doi l479-5876-6-50 This article is available from http content 6 1 50 2008 Mikirova et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Pharmaceutical doses of ascorbic acid AA vitamin C or its salts have been reported to exert anticancer activity in vitro and in vivo. One proposed mechanism involves direct cytotoxicity mediated by accumulation of ascorbic acid radicals and hydrogen peroxide in the extracellular environment of tumor cells. However therapeutic effects have been reported at concentrations insufficient to induce direct tumor cell death. We hypothesized that AA may exert anti-angiogenic effects. To test this we expanded endothelial progenitor cells EPCs from peripheral blood and assessed whether or not high dose AA would inhibit EPC ability to migrate change energy metabolism and tube formation ability. We also evaluated the effects of high dose AA on angiogenic activities of HUVECs human umbilical vein endothelial cells and HUAECs human umbilical arterial endothelial cells . According to our data concentrations of AA higher than 1 00 mg dl suppressed .
