Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Thymoglobulin, interferon-g and interleukin-2 efficiently expand cytokine-induced killer (CIK) cells in clinical-grade cultures | Bonanno et al. Journal of Translational Medicine 2010 8 129 http content 8 1 129 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access Thymoglobulin interferon-g and interleukin-2 efficiently expand cytokine-induced killer CIK cells in clinical-grade cultures z- r 2 1 1 2 Giuseppina Bonanno 1 Paola Iudicone 1 Andrea Maiiotti Annabella Procoli Annino Pandolfi Pi n Ỉ o 1 V i1 i2 l l rÍ2 C 1 Ir P A locc Dorillr P fOir iX Tif if ii Cr-TỉmHÌTỉ1 I I Diorolli2 3 Qar nim Dll f ollT5415 t Daniela rioiavanii 1 Maiia Coiallo 1 Alessandio reiillo 1 Giovanni scambia 1 Luca rieielli 1 seigio Rutella Abstract Background Cytokine-induced killei CIK cells aie typically differentiated in vitro with interferon IFN -y and aCD3 monoclonal antibodies mAb z followed by the repeated provision of inteileukin IL -2. It is presently unknown whethei thymoglobulin TG a preparation of polyclonal rabbit g immunoglobulins directed against human thymocytes1 can improve the generation efficiency of CIK cells compared with aCD3 mAb in a clinical-giade culture protocol. Methods Peripheral blood mononucleai cells PBMC fiom 10 healthy donois and 4 patients with solid cancei weie piimed with IFN-g on day 0 and low 50 ng ml z inteimediate 250 ng ml and high 500 ng ml concentrations of eithei aCD3 mAb oi TG on day 11 and weie fed with IL-2 eveiy 3 days foi 21 days. Aliquots of cells weie haivested weekly to monitoi the expression of representative membeis of the killei-like immunoglobulin ieceptoi KIR NK inhibitoiy receptor NK activating ieceptoi and NK triggering ieceptoi families. We also quantified the frequency of bona fide iegulatoiy T cells Tteg a T-cell subset implicated in the down-regulation of anti-tumoi immunity1 and tested the in vitro cytotoxic activity of CIK cells against NK-sensitive1 chionic myeloid leukaemia K562 cells. Results CIK cells expanded moie vigorously in cultures supplemented with inteimediate and high concentrations of TG compared with 50 .
