Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : A predicted protein, KIAA0247, is a cell cycle modulator in colorectal cancer cells under 5-FU treatment | Huang et al. Journal of Translational Medicine 2011 9 82 http content 9 1 82 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access A predicted protein KIAA0247 is a cell cycle modulator in colorectal cancer cells under 5-FU treatment Chi-Jung Huang1 2 3 Shung-Haur Yang4 Shih-Ming Huang2 Chih-Ming Lin1 5 Chih-Cheng Chien1 6 Yan-Chu Chen2 Chia-Long Lee7 Hao-Han Wu4 and Chun-Chao Chang8 Abstract Background Colorectal cancer CRC is the predominant gastrointestinal malignancy and the leading cause of cancer death. The identification of genes related to CRC is important for the development of successful therapies and earlier diagnosis. Methods Molecular analysis of feces was evaluated as a potential method for CRC detection. Expression of a predicted protein with unknown function KIAA0247 was found in feces evaluated using specific quantitative realtime polymerase chain reaction. Its cellular function was then analyzed using immunofluorescent staining and the changes in the cell cycle in response to 5-fluorouracil 5-FU were assessed. Results Gastrointestinal tissues and peripheral blood lymphocytes ubiquitously expressed KIAA0247. 56 CRC patients fell into two group categories according to fecal KIAA0247 mRNA expression levels. The group with higher fecal KIAA0247 n 22 had a significantly greater five-year overall survival rate than the group with lower fecal KIAA0247 n 30 p log-rank test . Fecal expression of KIAA0247 inversely related to CRC tumor size Kendall s tau-b p . Immunofluorescent staining revealed that the cytoplasm of CRC cells evenly expresses KIAA0247 without 5-FU treatment and KIAA0247 accumulates in the nucleus after 40 gM 5-FU treatment. In HCT116 p53- - cells which lack p53 cell cycle control the proportion of cells in the G2 M phase was larger 13 in KIAA0247-silent cells than in the respective shLuc control 10 and KIAA0247-overexpressing cells 7 after the addition of low dose .
