Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : MicroRNA signature of cis-platin resistant vs. cisplatin sensitive ovarian cancer cell lines | Kumar et al. Journal of Ovarian Research 2011 4 17 http content 4 1 17 RESEARCH JOURNAL OF OVARIAN RESEARCH Open Access MicroRNA signature of cis-platin resistant vs. cisplatin sensitive ovarian cancer cell lines 11 4 13 Smriti Kumar Arooshi Kumar Parag P Shah Shesh N Rai Siva K Panguluri and Sham S Kakar Abstract Background Ovarian cancer is the leading cause of death from gynecologic cancer in women worldwide. According to the National Cancer Institute ovarian cancer has the highest mortality rate among all the reproductive cancers in women. Advanced stage diagnosis and chemo radio-resistance is a major obstacle in treating advanced ovarian cancer. The most commonly employed chemotherapeutic drug for ovarian cancer treatment is cis-platin. As with most chemotherapeutic drugs many patients eventually become resistant to cisplatin and therefore diminishing its effect. The efficacy of current treatments may be improved by increasing the sensitivity of cancer cells to chemo radiation therapies. Methods The present study is focused on identifying the differential expression of regulatory microRNAs miRNAs between cis-platin sensitive A2780 and cis-platin resistant A2780 CP70 cell lines. Cell proliferation assays were conducted to test the sensitivity of the two cell lines to cis-platin. Differential expression patterns of miRNA between cis-platin sensitive and cis-platin resistant cell lines were analyzed using novel LNA technology. Results Our results revealed changes in expression of 11 miRNAs out of 1 500 miRNAs analyzed. Out of the 11 miRNAs identified 5 were up-regulated in the A2780 CP70 cell line and 6 were down regulated as compared to cis-platin sensitive A2780 cells. Our microRNA data was further validated by quantitative real-time PCR for these selected miRNAs. Ingenuity Pathway Analysis IPA and Kyoto Encyclopedia of Genes and Genomes KEGG analysis was performed for the selected miRNAs and their putative targets to identify .
