Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: and molecular size-selective detection of proteins using a chip-based and heteroliganded gold nanoisland by localized surface plasmon resonance spectroscopy | Hong et al. Nanoscale Research Letters 2011 6 336 http content 6 1 336 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Sensitive and molecular size-selective detection of proteins using a chip-based and heteroliganded gold nanoisland by localized surface plasmon resonance spectroscopy Surin Hong Suseung Lee and Jongheop Yi Abstract A highly sensitive and molecular size-selective method for the detection of proteins using heteroliganded gold nanoislands and localized surface plasmon resonance LSPR is described. Two different heteroligands with different chain lengths 3-mercaptopionicacid and decanethiol were used in fabricating nanoholes for the size-dependent separation of a protein in comparison with its aggregate. Their ratios on gold nanoisland were optimized for the sensitive detection of superoxide dismutase SOD1 . This protein has been implicated in the pathology of amyotrophic lateral sclerosis ALS . Upon exposure of the optimized gold nanoisland to a solution of SOD1 and aggregates thereof changes in the LSPR spectra were observed which are attributed to the size-selective and covalent chemical binding of SOD1 to the nanoholes. With a lower detection limit of ng ml the method can be used to selectively detect SOD1 in the presence of aggregates at the molecular level. Introduction Many proteins are capable of causing immune reactions and the presence of protein aggregates has been identified as an important factor leading to the lowering of immune tolerance 1-3 . For this reason the precise detection of proteins in the presence of other formulations with high sensitivity and specifity is essential for disease diagnostics drug screening and other applications 4 5 . The most widely used method for the detection and analysis of proteins and their aggregates in formulations is size exclusion chromatography SEC 5 . Although useful for the determination of molecular-weights of proteins its application to .
