An appropriate liquid-liquid phase separation is critical for the preparation of nanofibers and does not occur in all solvents that’s why selection of solvent and phase separation temperature is crucial for the formation of nanofibers. When the condition are favourable, liquid-liquid phase separation produce three dimensional fibrous structure with nano scaled architecture similar to that of collagen type I, and used in various biomedical applications. Advantage of the phase separation technique is that, it can easily combine with other fabrication technology (Particulate leaching) to design three dimensional structures with control pore morphology. It can also be combined with.
