We report the effects of stabilizing osmolytes (low molecular mass organic compounds that raise the midpoint of thermal denaturation) on the stabil-ity and function of RNase-A under physiological conditions (pH and 25 C). Measurements of Gibbs free energy change at 25 C(DGD ) and kinetic parameters, Michaelis constant (Km) and catalytic constant (kcat )of the enzyme mediated hydrolysis of cytidine monophosphate