The study of the pathogenesis of Shiga toxin-producing Escherichia coli (STEC) infections encompasses many different disciplines, including clinical microbiology, diagnostics, animal ecology, and food safety, as well as the cellular microbiology of both bacterial pathogenesis and the mechanisms of toxin action. E. coli: Shiga Toxin Methods and Protocols aims to bring together a number of experts from each of these varied fields in order to outline some of the basic protocols for the diagnosis and study of STEC pathogenesis. We hope that our book will prove a valuable resource for the clinical microbiologist as well as the cellular microbiologist