Across evolution, the signal recognition particle pathway targets extra-cytoplasmic proteins to membranous trans-location sites. Whereas the pathway has been extensively studied in Eukarya and Bacteria, little is known of this sys-tem in Archaea. In the following, membrane association of FtsY, the prokaryal signal recognition particle receptor, and SRP54, a central component of the signal recognition par-ticle, was addressed in the halophilic archaea Haloferax volcanii.