Wheatx-5 gliadin has been identified as a major allergen in wheat-depend-ent exercise-induced anaphylaxis. We have detected seven IgE-binding epitopes in primary sequence of the protein. We newly identified four additional IgE-binding epitope sequences, QQFHQQQ, QSPEQQQ, YQQYPQQ and QQPPQQ, in three patients with wheat-dependent exer-cise-induced anaphylaxis in this study.