Bone alkaline phosphatase with glycolipid anchor (GPI-bALP) from chick embryo femurs in a medium without exogenous inorganic phosphate,but containing calcium and GPI-bALP substrates,served asin vitro model of min-eral formation. The mineralization process was initiated by the formation of inorganic phosphate,arising from the hydrolysis of a substrate by GPI-bALP. Several minerali-zation media containing different substrates were analysed after an incubation time ranging from h to 144 h.
