Bothrops snake venoms are known to induce local tissue damage suchas hemorrhage andmyonecrosis. Theopossum Didelphis marsupialisis resistant to these snake venoms and has natural venom inhibitors in its plasma. The aim of this work was to clone and study the chemical, physicochemical andbiological properties ofDM64, an antimyotoxic protein from opossum serum. DM64 is an acidic protein showing 15% glycosylation andwith amolecular mass of 63 659 Da when analysed by MALDI-TOF MS.