Populations in Southeast AsiaandSouthChinahave highfrequencies ofa-thalassemia caused by a-globin gene mutations and/or deletions. This study was designed to find an efficient and simple diagnostic test for the mutations and deletions. A duplex polymerase chain reaction (PCR)/denaturing high-pressure liquid chromatography (DHPLC) was used to detect the mutations and deletions. A blinded study of 110 samples, which included 92 a-thalassemia samples with various genotypes and 18 normal DNA samples, was carried out by the methods. The duplex PCR products of the sample with known Constand spring mutation (CS)/aa, Quonsze mutation (QS)/ aa, and Weastmead mutation (WS)/aa DNA showed significantly different profiles, which suggests that DHPLC analysis at C can detect potential.
