A mixture 3-O- -D-glucopyranosylstigmasta-5,22-diene 1a and 3-O- -D- glucopyranosylstigmasta-7,22-diene 1b, 1’-O-benzyl- -D-glucopyranoside 2 and (8Z)-2-(2- hydroxypentacosanoylamino)octadeca-8-ene-1,3,4-triol 3 were the first time isolated from Polyscias serrata. Among them, compound 3 was the first time found in the genus Polyscias.