The results showed that four sulfate polysaccharides have branched structure, their chemical compositions are galactose, fucopyranose, fucofuranose, mannose, some other monosaccharides and sulfate groups in different positions. The sulfate polysaccharides from Sargassum aliqualium showed anticoagulant activity and higher sulfate content fraction has higher activity. | Journal of Science and Technology 54 (2C) (2016) 360-365 STRUCTURAL CHARACTERISTICS AND ANTICOAGULANT ACTIVITY OF SULFATE POLYSACCHARIDE FROM THE BROWN ALGA SARGASSUM ALIQUALIUM Bui Van Nguyen1, *, Tran Thi Thanh Van2, *, Bui Minh Ly2, Nguyen Quyet Chien3, Thanh Thi Thu Thuy3, Maria. I. Milan4, Anatolii. I. Usov4 1 University of Khanh Hoa, 01 Nguyen Chanh street, Nha Trang City, Khanh Hoa, Vietnam 2 Nha Trang Institute of Technology Research and Application, 2 Hung Vuong, Nha Trang, Vietnam 3 4 Institute of Chemistry, VAST, 18 Hoang Quoc Viet, Hanoi, Vietnam . Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Russia * Email: buinguyen810@; vanvvlnt@ Received: 15 June 2016; Accepted for publication: 24 October 2016 ABSTRACT Four fractions of sulfate polysaccharide extracted from the brown alga Sargassum aliqualium (Fucales, sargassaceae) were fractionated by using anion – exchange chromatography. Their structural characteristics were determined by desulfation, methylation and NMR spectroscopic methods. The results showed that four sulfate polysaccharides have branched structure, their chemical compositions are galactose, fucopyranose, fucofuranose, mannose, some other monosaccharides and sulfate groups in different positions. The sulfate polysaccharides from Sargassum aliqualium showed anticoagulant activity and higher sulfate content fraction has higher activity. Keywords: sulfate polysaccharide, Sargassum aliqualium, structure, anticoagulant activity. 1. INTRODUCTION Fucoidans are sulfate polysaccharides derived from marine brown seaweed containing mainly fucose and sulfate groups with other residues such as galactose, xylose, glucose, manose and uronic acids. Fucoidans were reported to possess various biological effects in vitro and in vivo such as anti-inflammatory, anticoagulant, antithrombotic, antiviral including anti-HIV, immunomodulatory, antioxidant and antitumor [1]. Vietnam has a coastline of about 3600
