Exopolysaccharide (EPS) production ability of Lactobacillus plantarumT10 was studied. The supplement of some sugars (lactose, saccharose, and glucose) gave the positive effects on EPS production of L. plantarum T10, in which the addition of lactose 4 % resulted in the most efficiency for EPS yield ( µg/mL). | Journal of Science and Technology 54 (4A) (2016) 40-47 OPTIMAL CONDITIONS FOR EXOPOLYSACCHARIDE PRODUCTION BY Lactobacillus plantarum T10 Tran Bao Khanh1, 2, Do Thi Bich Thuy2, Doan Thi Thanh Thao2 1 Faculty of Chemistry, Hue University of Science, 77 Nguyen Hue street, Hue city 2 Faculty of Engineering and Food Technology, Hue University of Agriculture and Forestry, 102 Phung Hung street, Hue city * Email: tranbaokhanh@ Received: 15 August 2016, Accepted for publication: 5 October 2016 ABSTRACT Exopolysaccharide (EPS) production ability of Lactobacillus plantarumT10 was studied. The supplement of some sugars (lactose, saccharose, and glucose) gave the positive effects on EPS production of L. plantarum T10, in which the addition of lactose 4 % resulted in the most efficiency for EPS yield ( µg/mL). The addition of % of yeast extract into culture medium with 4 % lactose provided the highest EPS yields compared to other nitrogen sources (peptone, beef extract), which were mg/mL. The optimal conditions for EPS production of L. plantarum T10 in MRS broth with 4 % of lactose and % yeast extract supplement were also studied. The results indicated that the highest EPS yield ( mg/L) was obtained in the conditions of 106 CFU/ml initial cell density, temperature of 35 oC, pH and 48 h incubation. Keywords: exopolysaccharide, cultivation condition, Lactobacillus plantarum, sugar. 1. INTRODUCTION Exopolysaccharide (EPS) are the biopolymer which is widely used in agriculture, medicine and food industry. In food industry, EPSs are used as food additives such as gelling agents, viscosifiers, stabilizers, emulsifiers, and water binders to improve food texture. In addition, there has been an increasing interest in exploiting the EPS-producing lactic acid bacteria for their possible biological activities including antitumor, immunostimulatory, cholesterol-lowering and antioxidant activities. Therefore, EPSs from lactic acid bacteria .
