The aim of this study is to isolation the compound in n-hexane extract from the aerial parts of the plant and then identify and characterize its structure. The isolation and purification afforded white needle-like crystals which were characterized on the basis of spectral data (IR, 1H‐NMR, 13C‐NMR and HSQC) and in comparison with literature data. The compound was concluded as a mixture of stigmasterol and β‐sitosterol. | ISOLATION OF STIGMASTEROL AND β-SITOSTEROL FROM OCIMUM TENUIFLORUM L. (LAMIACEACE) DANG THI THANH NHAN HOANG THI TRANG, NGO DUY Y Department of Chemistry, Hue University, University of Education Email: nhanhoasp@ Abstract: Some of the extracts were got from Ocimum tenuiflorum L. (Lamiaceace) by soaking and liquid-phase extraction such as n-hexane, chloroform, ethyl acetate and methanol extracts. The aim of this study is to isolation the compound in n-hexane extract from the aerial parts of the plant and then identify and characterize its structure. The isolation and purification afforded white needle-like crystals which were characterized on the basis of spectral data (IR, 1H‐NMR, 13C‐NMR and HSQC) and in comparison with literature data. The compound was concluded as a mixture of stigmasterol and β‐sitosterol. Keywords: Ocimum tenuiflorum L., stigmasterol, β‐sitosterol, extracts. 1. INTRODUCTION Bioactive natural compounds isolated from the plants still get the concern of many researchers. Ocimum tenuiflorum L. (Lamiaceace) is an aromatic plant that plays an important source for essential oils and used for food, perfumery, cosmetic and pharmaceutical industries [2],[7]. All parts of the plant, from leaves, seeds, roots and even the total plant, are used in traditional medicine for treating infections, skin diseases, cold, cough, malarial fever [3], [8]. Ocimum tenuiflorum L. extracts were shown to have antibacterial activities against E. coli, S. aureus and P. aeruginosa, anti-stress, antioxidant, antifungal, [2], [4], [8]. This is the first research of extraction and isolation from the aerial parts of Ocimum tenuiflorum L. from Quang Nam province. 2. EXPERIMENTAL . Plant materials Wild variety of Ocimum tenuiflorum L. was collected in Quang Nam province. The species identification was authenticated by Mr. Do Xuan Cam, botanical taxonomist, Hue University of Agriculture and Foresty. A voucher spicemen is preserved in the library of natural .
