Cold environmental conditions influence the growth and development of plants, causing crop reduction or even plant death. Under stress conditions, cold-inducible promoters regulate cold-related gene expression as a molecular switch. | Turkish Journal of Biology Turk J Biol (2017) 41: 552-562 © TÜBİTAK doi: Research Article Characterization of the promoter region of the glycerol-3-phosphate-O-acyltransferase gene in Lilium pensylvanicum 1,2, , 1, 1, 3 Li-jing CHEN * **, Li ZHANG *, Wei-kang QI *, Muhammad IRFAN , 1 1 1 1 2 Jing-wei LIN , Hui MA , Zhi-Fu GUO , Ming ZHONG , Tian-lai LI 1 Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning, . China 2 Key Laboratory of Protected Horticulture (Ministry of Education), Shenyang Agricultural University, College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning, . China 3 Department of Biotechnology, University of Sargodha, Sargodha, Pakistan Received: Accepted/Published Online: Final Version: Abstract: Cold environmental conditions influence the growth and development of plants, causing crop reduction or even plant death. Under stress conditions, cold-inducible promoters regulate cold-related gene expression as a molecular switch. Recent studies have shown that the chloroplast-expressed GPAT gene plays an important role in determining cold sensitivity. However, the mechanism of the transcriptional regulation of GPAT is ambiguous. The 5’-flanking region of GPAT with length of 1494 bp was successfully obtained by chromosome walking from Lilium pensylvanicum. The cis-elements of GPAT promoters were predicted and analyzed by a plant cisacting regulatory DNA element database. There exist core promoter regions including TATA-box and CAAT-box and transcription regulation regions, which involve some regulatory elements such as I-box, W-box, MYB, MYC, and DREB. Full-length and four 5’-deletion fragments linked with GUS vectors were constructed and transformed into Nicotiana tabacum by Agrobacterium-mediated transformation. .
